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Presentation Oral presentation
Title Diversity of Acidobacteria in Namibian soils and novel methodological developments in microbial ecology

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Short title Acidobacteria diversity in Namibian soils

Author(s) Pester, M.(1); Rappl, K.(2); Starke, I.(1); Baranyi, C.(1); Wagner, M.(1); Ludwig, W.(2); Loy, A.(1)

Presenting author Pester, Michael

Institution(s) (1) Dept. of Microbial Ecology, University of Vienna; (2) Dept. of Microbiology, Technical University of Munich

Keywords Diversity; Bacteria; Isotope signature; Microarray; Namibia

Abstract Excessive use of arable land, as can be seen in parts of the Kavango region in Namibia, leads to a loss of soil fertility and, subsequently, to decreased crop production. Microorganisms are a vital and dominant component of the soil ecosystem as they significantly influence the cycling of nutrients and carbon by decomposing plant and animal residues. We analyzed the diversity of Acidobacteria, which are major representatives of bacteria in soils, in respect to land use management. The three management types analyzed are acre, fallow soil, and pristine soil. The abundance and composition of Acidobacteria in the differently managed soils will be used to establish a bio-indicator system for soil health. In addition, we are developing cultivation-independent methods that will help to elucidate the ecophysiological role of Acidobacteria in soil. Progress made to develop a Stable-Isotope-Array will be presented; a promising new technique that allows tracing assimilation of a stable isotope-labeled substrate by many active microbial community members in parallel. This method would combine the versatility of Stable-Isotope-Probing with the high-throughput capacity of DNA microarrays and, thus, will enable analysis of a large number of soil samples in a reasonably short time.

Congress Topic Microbiology

Topic No. 7.3
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Ref. No. 517